---
title: How to Import a Manifest?
description: Learn how to import a manifest from the Settings menu, and use it when creating a project or as an in-silico panel in variant analysis.
---
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# How to Import a Manifest?
## Learn how to import a manifest from the Settings menu, and use it when creating a project or as an in-silico panel in variant analysis.
### What is a Manifest?
A **manifest** is a file in **.bed format** that contains the genomic regions of interest (ROI), which may be accompanied by their associated annotations.
The genomic coordinates are typically formatted as: **chr start stop** (tab-delimited). The manifest determines in which intervals of **genes and exons** variants should be called.
The manifest is required for project creation and determines **variant calling.** It can also be used as an in-silico panel during analysis.
### Supported Formats
The manifest must be a **tabbed .bed file** compatible with the genome reference **GRCh37** (hg19) or **GRCh38** (hg38).
**Option 1.** Your .bed file should contain at minimum three mandatory columns (*chr, start, end)* without headers*.*
For exemple:`chr1 12345 67890 chr1 70000 80000 chr2 15000 25000`
Column structure:
- Column 1: Chromosome (e.g., chr1, chr2, chrX, chrY)
- Column 2: Start position (0-based or 1-based depending on your convention)
- Column 3: End position
**Option 2.** A complete, extendable BED file is also accepted, with additional columns (e.g., gene name, exon number, strand information).
**⚠️ Technical notes:**
- The interval length should be greater than 1 bp. If you encounter an issue while uploading the file, contact SeqOne Support ([support@seqone.com](mailto:support@seqone.com)) to format your .bed file or add padding to the intervals.
- For gene coverage analysis (Tab GeneCov), exons that are not covered or are only partially covered by the design (\<80% overlap between the manifest interval and exon RefSeq boundaries) are considered non-targeted.
- For CNV analysis in the context of panel analysis, only exons with ≥ 80% overlap between the manifest interval and the exon are analyzed. If no gene exon overlaps ≥ 80% with the manifest interval, the gene will not be included in the CNV analysis.
#### Step 1. Access the Manifest Tab
1\. Click on the **👤 User icon** in the top right corner of the SeqOne platform,
2\. Select **Entity** **Settings** from the dropdown menu,
3\. Choose **Manifests**
4\. Click on the "Add Manifest" button
#### Step 2: Select the Reference Genome
You may upload manifest under **hg19** format (genome GRCh37) or **hg38** format (genome GRCh38).
#### Step 3: Upload the File
a. Click **"Add a File"**
b. Select the file you want to import from your computer
c. Once the file is imported (green checkmark), you can edit the manifest’s display name. We recommend choosing a clear, descriptive name.
d. Click **"Create"** to finalize the import
****
#### Step 4: Verify Import
Once imported, your manifest will appear in the Manifest tab with the following information:
- Manifest name
- Genome reference
- Upload date
- User who uploaded it
- Actions related to the manifest: download, deletion, and linked projects
### Using Your Manifest
#### In Project Creation
When creating a new project:
1\. Select **Sequencing method → Gene panel**.
2a. Choose your already uploaded manifest from the dropdown under Manifest.
2b. Select **"Upload new manifest"** and import the manifest the same way described above.
#### As an In-Silico Panel
During analysis interpretation:
1. In the **Variants** tab, open the **Filters** panel
2. Find the **"Panel in-silico"** filter
3. Select your manifest to restrict variants to those regions
---
Need help designing your manifest, adding padding to your intervals, or verifying your design before import? Our support team can help — reach out at [support@seqone.com](mailto:support@seqone.com).
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