---
title: How to Import Gene Lists?
description: Learn how to create and import a gene list using the required format, supported identifiers, and available upload or generation options.
---
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# How to Import Gene Lists?
## Learn how to create and import a gene list using the required format, supported identifiers, and available upload or generation options.
### What is a Gene List?
A **gene list** is a collection of genes identified by their:
- **NCBI Entrez Gene ID** (required)
- RefSeq transcript ID (optional)
- HGNC gene symbol (optional)
Gene lists are used for:
- **Restricted panels** in the Analysis drawer (locks analysis to specific genes)
- **Gene panel filters** in the Variants tab
- **Exome and WGS analyses** to focus on genes of interest
### How to Import a Gene List
#### Step 1: Access the Panels Tab
1. Go to **Entity settings → Panels →** sub-tab **Genes ;**
2. Click on **"Add genes list"**
#### Step 2: Choose Your Import Method
You have **two options** for importing genes:
#### Option A: File Import
**File requirements:**
- **Format**: .txt (tab-separated values)
- **No header row**
- **Three columns** (only the first is required):
**Example .txt file content:**
```
672 NM_001126112 BRCA17157 NM_000546 TP532263 NM_000142 FGFR2
```
**Steps:**
1. Prepare your .txt file with gene IDs
2. Click **"Add a file"**
3. Select your .txt file
4. Click **"Save"**
#### Option B: Generate option
**For quick lists or small panels:**
1. In the **"Gene symbols"** field, enter a **comma-separated list** of gene symbols:
```
BRCA1, BRCA2, TP53, EGFR, KRAS, BRAF
```
2. Click "**Generate"** to automatically retrieve the corresponding NCBI Gene IDs. Copy the resulting table into a file, excluding the header row.
3. Once the file is ready, follow the same steps as in Option A:
4. Click **"Add a file" **
5. Select your .txt file
6. Click **"Save"**
### Size Limitations
⚠️ **Maximum 1000 genes** (the limitation is coming from genenames.org website directly)
### Format Requirements
- **File format**: Must be a tabular file (.txt/.tsv) without column headers
- **Required column**: First column must contain **NCBI Entrez** gene IDs
- For **Mitochondrial genes only**, please use the **ENSEMBL IDs**
- **Optional columns**:
- Second column: RefSeq transcript ID
- Third column: HGNC gene symbol
### Known Issues 🚨
- **No warning message**: Files are generated without automatic verification or validation of gene symbols, so unrecognized genes are not reported
- **Header row in generated files:** Files created via the "Generate" option currently include a header row, which must be removed before uploading — the platform requires files without a header row.
---
Need help designing your gene list or choosing the right identifiers? Our support team can help — reach out at [support@seqone.com](mailto:support@seqone.com).
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